let’s take a look at the principle of pregnancy testing, which is made using this single clone antibody. I’d like to find out how this red line is made. In order to become pregnant, the egg in the ovary is ovulated like this in the After the ovulation, the fallicle changes to the colpus luteum. And when the egg and sperm meet, it becomes a fertilized egg, and the fertilized egg goes through an ovary. The fertilized egg passes through cleavage and goes through oviduct for about a week to the utrus. So when the embryo implants in the inner uterine wall, it says she’s pregnant. Implantation is the most important part of pregnancy. Even if it’s fertilized, you can’t get pregnant if you can’t be implanted. When the embryo implants, it’s buried in the inner uterine wall The outermost membrane of the embryo is called the nutrient membrane, which forms a villus. As the villus progresses, the placenta is formed, but first, the hormones start to release in the vilus. And that hormone is called HCG. HCG is Human Chorionic Gonadotropin, HCG is a hormone that comes from human villus and stimulates gonad The HCG comes out of the villus, and the HCG goes to the ovary. An ovary is a gonad HCG goes to the ovary and stimulates the corpus luteum in the ovary. When HCG stimulates the corpus luteum, estrogen and progesterone are released from the corpus luteum. The secretion of estrogen and progesterone is to keep the inner wall of the uterus thick. The reason why the inner uterine wall should be kept thick is to help the embryo grow well. At best, the corpus luteum can survive up to three months. After three months, the yellow body almost degenerates, so it no longer secrete estrogen and progesterone. Instead, after three months, the placenta is completely formed, so the placenta itself produces estrogen and progesterone. corpus luteum does not need to secrete estrogen and progesterone. If corpus luteum degenerates, there’s no need for HCGs to stimulate it. So the amount of HCG is getting smaller. HCG can come out as soon as it’s implanted. But it’s a very small amount, so even if you’re pregnant, until about two weeks after the coitus, the signs rarely appear on the pregnancy test machine. HCG will be the most prolific in about 10 weeks. We can make sure we’re pregnant at about 10 weeks. Between two and three months after fertilization, the largest amount is released and then the amount is reduced. There’s antibodies in the pregnancy test kit. I’m going to take a look at how it exists When you look at the pregnancy test kit, you see an absorption bar where urine is absorbed, and you see three red lines on the bar. These lines contain mainly antibodies. The first red line is not visible to us. Because the first red line is hidden by a silver canister and is hard to see. Let’s take a look at what’s attached to each of these three parts This front part becomes an absorption rod. The direction in which the urine travels is to the right. The first line is blinded by a barrel, but there is an HCG antibody. HCG antibodies are antibodies that bind to HCGs with colouring matter. The first of the two red lines that appear in our visible examination window is the line that indicates pregnancy. If there’s a red line here, it means you’re pregnant. What’s shown in the back is the end-of-search display. If the part here is not marked, the examination is not complete. It means that the urine didn’t arrive here. This red line is what happens when the urine arrives. So let’s look at what’s in this red line. HCG is a hormone that only exists when you’re pregnant. And what’s in this first column?
There is an HCG antibody. It’s an antibody that binds to the HCG. And this red material that’s attached to it is a color substance, which, depending on the situation, can be colored When the HCG engages with the antibodies on the first line, it becomes an HCG complex. Among the two lines appearing in the pregnancy display window, the first line contains antibodies that bind to the HCG complex. The HCG complex joins the antibodies. This is an antibody to the HCG complex binding. It’s Y-shaped, so it’s an antibody. This is the HCG antibody, and the second line of the pregnancy display window is the antibody that binds to this HCG antibody. The HCG antibodies have a colorimetric substance, so when urine flows, they are naturally combined with the antibodies on the second line of the pregnancy window. If we get here, the red line is bound to appear. Let’s take a look at the pregnancy. If you’re pregnant, there’s HCG. So this HCG is going to create a HCG complex. And then the urine moves to the right, HCG composites bind to antibodies on the first line of the pregnancy display window. The colorimetric material then causes the first red line to appear in the pregnancy In the course of urine, these non-combined colourants also flow along the urine. These are red colored when they meet the antibody in the second line of the pregnancy window. So, if you’re pregnant, you’ll have two lines when you’re done. If you don’t get pregnant, the first line of the pregnancy window doesn’t have the HCG antibodies to join, so you don’t have the red lines. Antibodies with colorimetric material come into the second line of the pregnancy sign and create a red line. So if you don’t get pregnant, just one line is made. This is the principle of a pregnancy test using a single clone antibody.