Breeding German Shepherd Dogs : What You Need to Know (2018)

way now guys it is really time to discuss breeding german shepherd dogs in 2019 and really discuss what you guys need to know and the great thing about this topic is you know we're going to talk about my favorite topic in the world which is canine reproduction after all that's what I'm an expert in basically and it's kind of interesting you know people always assume that dogs all work the same and when I met you guys when you know we do lectures with my colleagues like Emily and period at work I I say something and then there's always somebody in the room who will tell me well you know what Jack this what you just said does not apply to me because I braid XYZ I know always I don't always agree on that but it is true that some breeds do a specificity and in canine repro one thing I can tell you is that German Shepherds sure do they do have unique reproductive features so to speak and you see when I stopped in my career at the vet school in Paris we were basically working with 10 to 12 different German Shepherd bitches on a weekly basis so I have the opportunity to work with many German Shepherd breeders I have lots of stories to tell lots of info to share with you guys but Emily didn't mention it but I am guys if you attended to some webinars in the past probably know that I'm quite talkative so for times sake tonight we will only stick to three clinical cases and if you want more you know what at the end we will add a Q&A so you will add the opportunity to ask me any questions you want related to canine repro in German Shepherd so without further ado let's dig in and let's start with our first clinical case I want to tell you about the story of Tanya Tanya was a two-year-old German Shepherd bitch I first saw in the spring of 2005 in Paris that doesn't make me feel any younger but you see her owner was about to greet earth for the first time she was a preemie Paris bitch and you know in France nearly all my clients breeding German shepherds when they wanted to bring their bitches well you know what they would go to Germany think there's a song called to Germany with love but I'm not sure it across the ocean but anyway but Germany one thing even to know for his French people Germany is a long long way from friend so very long drive in European standards at least and I know I when I say that it keeps making you guys laugh in North America but hey look at the map you are visibility waste model so Germany is a very long way from Trent and on top of that in Germany my German Shepherd breeders would only be allowed one breeding the code is because this a one-shot breeding of course they wanted to optimize your chances here they wanted to make sure that if they were driving to Germany for one reading that they maximize their chance to add status and for that you know today in the beach there is one thing you need to know one thing super important in order to maximize your chances in order to optimize fertility and productivity which is the number of puppies in the litter you need to know the day of ovulation in the pitch and they are plenty of things we could say about that one thing I want you to remember there today no better way to do that then what we call progesterone testing this is still the gold standard in canine reading so this is a procedure we call timing of ovulation and I could spend easily 1/2 hours discussing it but you know what guys in the past we did a webinar on this very topic and if you're interested in what I will do right now live I will share with you on the chat right here took two quick everything is done live time so I will share with you this link to a presentation we did on this topic so if you are interested in you can check this presentation later on we view it it will cover all the most common techniques that exist today around the world to do timing of ovulation in canines but at the end of the day the gold standard the best technique remain remain remain doing conscious road tests drawing blood during the season determining the level of progesterone and with that determining the day of ovulation and this is today the goaltender and that's why those German Shepherd breeders we are coming to see us in Paris because they wanted us to do these tests to tell them ok this is when you need to go to Germany to with your bitch and you see when we do those consultations in canines we always start with something we call an animal anamnesis which basically means we collect data and info regarding the history of the beach and one key question I would always ask the owner here how often is the beach in season how often does she cycle in street Oh believe me that's always the first question we start with when we have those kind of discussion and you see Tanya owners was definitely not too much of a masters of the math version but he must have known that the question was coming you know because as soon as I act in the question well he gave me this so you see this is a copy of Tanya's records that I used for this presentation but you see she had a first season on in December 2004 and then she was in season in April in August in December and I was seeing her at the vet school in March of 2005 so tell me guys what do you think he what do you think if one of your bitch was cycling like this what would you think if she was in season that if she had this pattern what would you think tell me on the chat if one of your bitch if this is what you had recorded on one of your bitch on one of your breeding Beach what would you think tell me on the chat what's your opinion here you see she she started offseason December 2005 and then she cycle she was in season in April August December and then again March 2005 tell me on the chat what do you think here if that was one of your bitch what would you think about that and don't be shy guys remember I'm the one not speaking English here so somebody stays over Thank You Amit it seems like a lots of seasons often every hormones too close for relation okay I see all the answers showing up hey guys one thing first thing I want to tell you about this document what kind of document I should say but this page here you see it brings up a very important point we always discuss with you the burritos you need to record when your bitches are in season you must write those write down those day oh we call them in an excel sheet if you're picky but you say this is good practice and I would even say this is kind of mandatory because when you visit a repro vet like me this question is really on top of the list sometimes people tell me or she is cycling on a regular basis she's regular this is not an answer accurate enough for me if we go back to to the notepad Tanya Tonya's on your shoulders if we go back to this nut bag you see in fact this is what we would say this is what's happening in fact here so you see what we call the interest interval the interval between two seasons hormones hormones hormones three months look at that and now guys tell me if one of your bitch was fighting that often what would you think of here nothing to worry about maybe an ovarian cyst maybe this bitch is suffering from what we call details Taylor maybe hypothyroidism what would you think of if one of your breeding bitch was cycling like Tonya every four months every basically every four months what would you think of tell me on the chat what's your opinion here you five four three two one okay fifty percent of you paying for the things that we are dealing here with an ovarian cyst so you think eyes in canine in canine we prove one thing which is key is what we call regularity I'm sure you looked at some textbook so you might have veterinarians like me mentioning it during lectures which is typically cycle twice a year every six month that's typically what we see in average however here German shepherds you know what they are quite unique because in this breed it is really not uncommon to see bitches cycling every four to five months so that's something I would expect especially in German shepherds so I've seen German shepherds tightening every 4 moles which mean three times a year and adding normal size litre adding no fertility issues so as long as they are regular here as long as they are cycling every four month and they are regular typically usually there's no need to worry it might be totally normal here but you see when I was seeing Tanya when I looked at the paper I was okay with those number of hormones hormones problems okay she was regular but something caught my attention this one here three moms Tremont even if it's a German Shepherd bitch can cycle every four months this three-month interest rates interval I really seem to shock to me and when we see this we refer vet immediately think of Valley insist especially ovarian cysts that are producing estrogen because one of the clinical symptom will expect to see in those cases is what we call mean for mania a bitch that suddenly start to cycle more often eventually every three months so this is what we would think of a meta P here so if we think of an ovarian cyst how would you check that out how would you confirm the fact that the beach is suffering from an ovarian cyst would you do an abdominal palpation trial to palpate the abdomen to try to fill the cysts would you draw blood and do a blood analysis would you do an ultrasound or would you use Emma hi so what would be the best tool here to diagnose and advances in a bitch you five four three two one and I think we all agree a hundred percent of you believe that we need to use you to sound and I'm a big fan of your true self I think this is a fantastic tool and when we work in k9 we pro honestly I was doing infrasound every day several times a day because that's one of the best tool that give us access to what's happening on the things on the genital tract especially the ovaries and the uterus definitely the best technique to check if there's an ovarian cyst here and always something we will consider off your hand when we deal with any infertility issue in canines or when we deal with the case like Tanya here that apparently the interest rates interval is shorter than expected when the bitch is cycling more than often so in the case of Tanya we went straight to the ultrasound machine we check the ovaries and in German Shepherd which actually have great pictures great definition because in your breed we don't have a lot of abdominal fat so the ultra the ultrasound definition is usually very good especially with the machines we are this day and you think Tanya that's what we could see so this is beautiful guys so let me show you so you see this is the overly right here and inside its ovary maybe you guys see those black structures here which are roughly 0.5 centimeter in diameter those are what we call ovarian follicles this is a beautiful ovary beautiful other way with big follicles and in fact in those follicles in those things I highlighted in green here this is inside what you are the eggs that I'm going to be related and later fertilized by the sperm of the male so this is absolutely normal this is what I expect to see in a bitch that is in season but is getting closer close to ovulation there's no valiant is here well violence is usually look like this so you see those structures are much bigger typically and alliances is we will describe an evaluation sit as a round shaped structure like this big black bowl that you can see on the ovary which is more than one centimeter Indiana and this is sitting basically inside or only over it to see we have three of them here those are all very insist we didn't see that in Tonya in Tonya again look see of a result the other is beautiful it's right here it's really beautiful they have no structure that is that big that will make me think there's an ovarian cyst nothing that could explain the fact that she is cycling shared the three months in service to think about so you see in this situation there was nothing abnormal on the ovaries so no variances my conclusion was that you know what nothing abnormal then let's start doing the timing of ovulation and then when we will have detected when we would have detected the day of ovulation the witch will go to Germany for breeding the story doesn't end here obviously later that week I we discussed that case with my former boss during our weekly round you know and to tell you the truth I thought we would go over this case very quickly because well found it to me like a regular timing of ovulation in a premie thomas bitch i mentioned that i found the interest rate interval to be a bit shocked but i did an ultrasound and everything looked okay there was no variance it and you see as soon as i finish my sentence my former boss stood up and he went straight to his computer we went to publish the scientific articles search engine all scroll out use across the world and he uploaded this this article that was talking about short interest interval infertility in bitches with shop interest rates interval we applauded that and he later he told me about something we called soft and astra syndrome and he added on top of that now as you see we often see this problem in German Shepherd ditches okay so according to you what might happen here what might be the issue here if Tonya is suffering from which shop understood syndrome we have no problem my boss was originating maybe she's not going to ovulate maybe she's not going to get pregnant or maybe she's going to abort according to you you she's suffering from a shop and a stress syndrome what might be the issue what do you think is going to happen here you five four three two one okay so let's see the results okay so 50% of you guys believe there will be no gestation for you guys in this situation Tania would have probably ovulated she will die populated normal eggs that would have certainly be fertilized by the males Promotora both eggs would have done in two embryos exactly what we expect them to do except that those embryos might not have been able to attach to Tanya's uterus because at the time of the pregnancy diagnosis three four weeks after the breeding we would have seen nothing because of this shot under stress syndrome thing so let me explain a very specific feature of the canine uterus during your season the bitch is going to ovulate and the other reason will bear what we call control idea those structures that will produce progesterone for around tumor whether the bitch is pregnant or not and after two months you see they have no more project on the projects equation drops and the bitch gives birth or basically enters what we call a mistress the this period where when there is no almond on impregnation however something is going to happen at the level of the uterus after most amount something we call uterine involution let me I like this uterine involution to make it super short this is kind of a regenerative process that is necessary for the uterus to accept a new pregnancy and this regenerative process basically takes two to three modes so if the bitch cycles every three months there is basically not enough time for this uterine involution for this regenerative process to happen you see in Tania's case because of this three-month interval should interest interval the uterus didn't have enough time to go back to its initial state she cannot get pregnant it's not possible everything else seems to work well but because this uterus is not back to its original state so to speak even if there are embryos they cannot attach to the uterus and she cannot develop the gestation so there is good news is here or whether something can be attempted to fix the problem and in fact it was described in this paper I showed you before you see here they were talking about projecting treatment for infertility in bitches who shot into our stress interval and they used industries in this in this study they used a drug called magistral acetate which is what we call a progestogen a molecule that acts like progesterone and in fact we use that because at the very beginning of the cycle it will inhibit the development of the ovarian follicles understand that is going to stop is going to stop the season anemia paper they use these protocols in ten bitches suffering from this problem and then they bred those ten bitches and guess what ten which is ten of those ten out of ten were pregnant so the goal here is super simple you stop the season at the very beginning so that it'll you alone more time for the uterus to regenerate and unfortunately when we use this protocol we need to use it at the right time we shouldn't use it too late in the season of the witch because then there's the risk to induce potentially a pyometra so it's very important to really really use what respect all the different steps of the protocol so in the case of Tanya she was already too far advanced in our season we couldn't do that we couldn't use it at that moment so you know what we decided to wait we decided to wait for the next season which apparently might come in the next three months which was the case she was in season three month after the beginning of this season and when she had this new season when we did the major solicitor to acetate treatment the season stopped and she was bred at the following season again she had a signing of a relation she went to Germany to for a single one-shot breeding and guess what she adds puppy happy handling but if we had bred herb first time I saw her that probably wouldn't have been decay because again of this shop under stress syndrome and this shop under stress syndrome is something I have mainly seen actually man see I've only seen it in German Shepherd dogs and that's why I believe this is something good for you guys to remember because you are definitely in a breed that is predisposed for this let's now move to our second clinical case and this time we'll speak about angel the story of angel the 3.5 year old German Shepherd bitch I saw in consultation in Paris again a long time ago and she was this time we fell to us for infertility so you see when I first discussed this clinical case with the owner this is what he told me he told me that angel add regular season she was in season roughly every five to six months which is kind of the norm she was bred at all a season and she was read on German in Germany on day 12 of her season the mail that they use was proven to be futile yet successfully breed bitches before and after this breeding they did a pregnancy diagnosis by abdominal palpation the thought she was pregnant but not appease web delivered after that and when they came to see us at the vet school in Paris she had started off season roughly a week ago so when I saw her in consultation I could also see that you know she was in optimal body condition and I want to reiterate that this is something super important when it comes to breeding again some people tend to forget that but we want the bitches before they are bred when we breed them we want them to be in optimal body condition because fat tissue is not only for energy storage it also produces hormones that can interfere with the normal development of the normal also with the normal hormonal control of the reproductive function and I was confused because I was looking for this blood but I wanted to share with you on the chat but to explain you guys more about what I mean here but optimal body condition is really key and that should be your priority you want the veg to be optimal body condition before breeding her she was okay she was in super super good else absolutely nothing abnormal from what I could see here so you see this is our history she was infertile what should we start with here should we do progesterone test should we think of an intrauterine insemination should we do a Brucella canis test with the top of canine brucellosis or should we check for hypothyroidism what do you think what should we do here what should we start with you five four three two one okay let's see what you guys answered so everything nearly fifty people well 40% of you mentioned we should check for hypothyroidism and you know what's kind of interesting because this is something I hear very often all the bitches infertile we need to check out hypothyroidism one thing I can tell you and we have studies to back this up and I might have a blog but I need to look for it but I'm not going to do that right now but one thing I can tell you for sure when a bitch or male dog is suffering from hypothyroidism the first reason why you will go to see a veterinarian is not infertility you will have other clinical symptoms you will ask maybe skin issues or overweight in the beach because she keeps getting on and getting we're putting on weight but it will not be infertility infertility can be caused by hypothyroidism but usually doesn't come along it it comes with all the other clinical symptoms if the only symptom is infertility studies tell us and I've seen this at the vet school it's usually not it has nothing to do with i populism and it's very often overestimated because in large dogs we have lower levels of thyroid hormones so very easy to miss interpret the results your but remember well remember look at the history here you see previous age she was bred in Germany on day 12 so she was born on day 12 so that's why I would definitely suggest here to start with project one test for timing of ovulation and next season we already mentioned it a little bit earlier but this is the best way to determine the day of ovulation and I keep mentioning you know in my lecture that you guys work with animals that are very unique in the field in the field of animal repro bitches produce progesterone before ovulation it's not nelly the only species where we can use progesterone to detect the day of ovulation because it's produced before it and they will oscillate outside eggs that are not yet mature they will become mature to four days sometimes five days after ovulation which is super convenient for you guys because it means that you don't when they are populated you add time to drive to where the male leaves you have time to call your friends so that they can ship clothes and semen or shows and maybe not that she'll semen to your place so it makes it much easier for you to schedule all the different events those are very unique features of canine repo and that being said they are unique but in many many very whole text books and to be fair on many many Facebook forums – you will read that people recommend to greet the bitches between 12 to 15 days after the beginning of the season because this is when they are ready this is what people keep telling me they are always ready between 1215 days you know what guys we know better today we know that bitches can ovulate earlier or later and that's the reason why well you see this this is not always correct that's the reason why miss timing of the day of breeding is the most common cause of infertility in canines because basically to see is very simple sperm cells cannot meet the eggs and basically November your not pregnancy but true for voltages but it's even more true of the truth we say more to a truer but you guys can correct me on the chat it's even more true or truer in German Shepherd dogs because of this okay I know it's written in French but guess what Canada is a bilingual country guys so it's time to brush up your French I'm just kidding I'm going to translate this but this is a study we did in 2006 at the vet school of Paris and basically we studied the play ovulatory period in the German Shepherd itch and again this was a bit we were seeing a lot in consumer type of w we're seeing a lot in consultation and of dogs German Shepherds are known to have what we call long pro illustris so pro estrus is the first phase of the season and it's definition is mainly behavioral you know and it means the bitch is in season but she does not accept mating very behavior oriented definition because in real life I'm sure you have seen which is exciting we be accepting being read any time during the season right but what is interesting is that in this study that we did at the vet school it showed us that in fact 30% protein one bitch out of three which is kind of huge 30% of German Shepherd bitches will ovulate between day 16 and 25 after the beginning of their season I have even seen some of them oscillating more than 50 days after the beginning of their filaments in which she ovulating on day 40 she would she was bred on day 32 and she had 12 apiece so you see which German Shepherd bitches are known to ovulate later in their cycle and let's take Angela scale let's imagine that she had ovulated on day 20 of your season which again is really not uncommon in German shepherds that means that the optimal fertility window would be between data 22 and with day 24 because again the other sites will become mature choose days and we stay mature two to four days after ovulation she was bred on day 12 so on day 12 well what we know is that the half-life of the scrum is basically two to four days so which means that we would still have sperm around until day 16 after the beginning of their season and she related on day 20 should have been ready day 22 day 24 so you see by day 16 clearly would be nothing less basic maths the sperm cells here might not have been able to fertilize the eggs in this scenario which again is highly probable here because it's a German Shepherd dish that was sired that was bred day 12 after the beginning of the season so it varies a lot but in German shepherds we know for sure they have this long foresters that is very specific to the breed so what else would you recommend here that so we are agreed we need to do a timing of relation we need to do progesterone test what else should we do should we do an intrauterine insemination should we do a Brucella canis test as for canine brucellosis should we do ultrasound prior to breeding should we check for hypothyroidism I will give you my take on that one so but what do you think what should we recommend here you five four three two one okay that's kind of interesting most of you went for intrauterine insemination and well one thing I wanted to mention first you see the brutal I can attest canine brucellosis that's always something I would always recommend in any case of infertility which is infertile male dog is infertile we want to check cocaine and brucellosis because this is a very severe infectious disease that can impact fertility essentially in canines and you really want to rule this one out especially today we have reports of canine brucellosis outbreaks in certain countries in certain states so we want always we always want to rule this one out always that's the priority that's the first thing even if it's not very common fortunately we want to rule it out but clearly one of the thing I would consider here would be an intrauterine insemination you see that is a beautiful cervix of the beach and basically the cervix is this structure that separates the vagina and the uterus so the sperm cells had to go through this to get inside the uterus and here it's like they have arrows pointing telling you the entrance is right here this is a beautiful picture of the cervix no doubt about that and this one this picture was taken on an English Bulldog but to be fair we are very similar cervix in in German shepherds and we would like I would like to perform what we call TCI transfer tickle insemination and intrauterine insemination so that I can pass the catheter through the cervix and deputies the semen inside the uterus I would like to do that for two thing because first it would allow me to check the quality of the semen of the male at the time of breeding because the fertility of the male the poor semen quality is the second cause of infertility in canines and well if I can I would always prefer to check it but don't you worry more about this in the next clinical case we are going to discuss any of us human is a good quality again I like to make sure I can deposit the semen inside the uterus I want to bypass the vagina so that it increased it will maximize the number of sperm cells that can reach the outside and the other reason is you see the vagina of the bitch is extremely long the end because of the blood that is produced during the season well some of the blood can accumulate and sometimes macerate which is a perfect medium for bacterial growth and that's why large which is German Shepherd which is included are more predisposed to vaginal infection during the season however you know this is a German Shepherd bitch we are talking about here so in Europe as I told you AI is not something that is usually done they go from natural breeding most of the time because that's what is considered the gold standard in this breed so I offered this to the owner of Angel and he told me no no no way I want there to be bred in Germany so we won't do an artificial insemination we couldn't do an artificial insemination we wouldn't we couldn't do it with TCI on Angel but at least I would always recommend to check if the bitch is not developing a vaginal infection during the season because of what we just did without the length of the vagina et cetera I have a question for you guys what do you think is the best way to check if Angel is developing eventually the bacterial infection is the general infection what's the best way to check she's developing a bacterial infection should we do a blood culture through the vagina smear SH with the vaginal culture should we do the genuine endoscopy what's the best way according to you to check if she's developing the general infection here you five four three two one let's take a look at the result ah that's very very interesting guys eighty-seven point five percent of you are telling me that we should do the general culture so we should do a bacteriology to check in they are bacteria growing in the vagina really interesting you know you see when I saw Tonya Tonya in consultation before we started doing the timing of the relation we will always do a clinical exam and when I perform clinical exam in greeting bitches I always check obviously the vulva to check it out there's no abnormal to join or discharge so I know this is a very old picture it's kind of blurry but in 2005 guys we didn't have iPhone at that time but here it was no covenant without discharge the discharge coming out of the vulva was normal normal imaging discharge I would expect during the season of the beach so we did a vagina Slayer because the Jango smear is a very important tool we use when we do timing a lot relation but we also use it to check what's inside the vagina so you see those complex like cells they are telling you that the bitch is in what we call Easter's which means that is very it means very high secretion of estrogens but it means that we are getting close to the ovulation the interesting part is both kind of vaginas meals you can see them from ten days before ovulation to ten days after ovulation but anyway what we are looking for in those vagina smears we are looking for something we call neutral files which are cells that are not gross of inflammation that will tell us that they have something wrong going on and probably an infection in the vagina of the witch but here there is absolutely no neutral pipes this is totally normal we just see qualified vagina cells which are those cells here absolutely no sign of inflammation and infection and because I was super lucky when I was at the bed school I had access to endoscopy so I did a vaginal exploration and those complete the small camera that I can put in the vagina of the bitch and I can either look at the vagina on bukoza and this is what I see and look it's pink it's clean is simply beautiful really beautiful here so here in turn in angel cake there is absolutely no sign of infection absolutely no sign of infection so you see if I have done the bacterial culture this is what I might prove the other thing I might have found we coli in heavy growth I might have found streptococcus G in AV growth I might have found another bacteria called Pasteurella me to zero in every growth and I could tell I can tell you something that would have been very very confusing because I would have found the bacteria growing in the vagina but you've seen the pictures everything was everything looked absolutely normal to bacteria bacterial to general cultures is something you know people often use when dealing with infertility any don't get me wrong it can be an interesting tool but it is an interesting tool only when it is associated with clinical science and this is a publication that was a paper that was published in 2016 I guess but here it tells us that the general bacterial flora increases which is rollin through tivity and I want to put them here antibiotic treatment before mating on the basis of positive culture yet in the absence of clinical science seems to be unnecessary so I'm just raising a warning here you know that today antibiotic resistance is on everyone lips I keep repeating that during my webinars I think but if you do a bacterial culture in a breeding bitch I can guarantee you one thing it will always come up positive we did that for five years until we got access to endoscopy and we realize that everything was normal we will always find something we might find those three germs I mentioned Rico lice Cryptococcus G Patrignano Sauceda in AV growth in a normal pagina with no infection at all remember that the test is always only as good as its as its interpretation so beware because here positive doesn't mean infection and it certainly does not mean that the bitch needs to be treated with antibiotics which turns out to be in fact way too often the the go-to solution in those situation so you see in the case of Tonya everything was normal we did a timing of ovulation as we said because we have some doubts about the way she was bred when she was bred the first time we did the timing of ovulation we determine when she was supposed to be bred and she went to Germany to be bred with AB I thought I'll nail so okay breathing okay what do we do next we wait and cross fingers should we schedule a blood test the week later should we schedule an intra sound in three weeks should we schedule an x-ray in seven weeks what should we do next after the breathing should we just wait and see what should we do here what would you do because again remember this bitch add a history of infertility five four three two one okay so I see that most of you guys share my love for ultrasounds that's great well you see one thing that caught my attention in ten years case was this farm right here in Kenya sorry this one is angel it's not Kenya we already talked about tanning it's this part here this is a pregnancy diagnosis by abdominal palpation and okay abdominal partition is not the best way to confirm that the bitch is pregnant I myself and my share of failures with this however I knew angels breeder was quite good at this using this for more than 13 years and you know usually when he was coming to see me at the vet school and you were telling me hoon I think she's pregnant he was really wrong and it is definitely possible that angel was pregnant and dealt with what we call embryonic resorption so the question I get quite often is c'mon doc how come this went to notice if she was pregnant and she lost the puppies there should have been some sort of clinical side mimicking an abortion here they didn't see anything they thought she was pregnant and there was no puppies so here's an important rule you guys need to remember to certify their rule in dogs any pregnancy arrest that occurs before 35 days of gestation will go totally unnoticed because the embryos will basically will be literally digested by the uterus so to speak so in an infertility k it says it therefore means that you must do an early pregnancy diagnosis with the conventional machine this is something we can easily do especially no showmanship a bitch around three four weeks after breeding and this is what we did in angel we did an early pregnancy diagnosis by ultrasound and this is what we saw look here there's even an arrow pointing to this thing here that might be too hot for the puppy probably ditching when you see that this means congratulations you succeeded there are puppies we didn't see anything like that you see this is an embryonic resorption this is an embryo but there's nothing inside the it's totally empty so we know that something started but it couldn't go through it didn't fully develop and here's another picture for them Briana quiz option we didn't see that in angel cake in angels case we didn't see any pictures of embryonic resorption we only saw pictures of embryos we were super happy you know everything looked okay she had puppies so the owner was happy he was I can tell you who's not bouncing up and down in in the ultrasound room but again the story doesn't end here the story so is Odin Elia because remember Henschel history the breeder thought she was pregnant but she didn't deliver anything everything looks normal in dirt you sure sound but because of the history and also because of the fact that angel is a German Shepherd bitch we also did raw blood to save it again we are said progesterone we did a progesterone set at the time of the pregnancy diagnosis because one thing I know and one thing I want you to know about German Shepherd bitches this is verb breed in which we most often diagnose something we call new tails failure and this one is very very important for you guys to remember and by the way retail failure tell me what is this no idea uterine disorder and volume these other infectious disease what is luteal failure a problem we see quite often well quite often that is most often reported in German shepherds and in fact we also call it a little insufficiency what is that no idea uterine disorder and volume these other infectious disease five four three two one so okay most of you enter a user user means disorder uterine disorders are quite common and definitely a very important cause of infertility in witches but you see gluteal failure is not a uterine disorder little failure is in fact is in fact it corresponds to the lack or no progesterone equation by the cocktail area which you get are those structures on the ovaries after a during gestation no progesterone to in gestation or not enough of it means one thing nope gestation because gestation can only be maintained with the presence of progesterone if you don't have protector on you as no gestation and retail failure means that the ovaries for the reason we don't clearly understand today are no more producing cholesterol or are not producing enough progesterone so we did this with draw blood in angel and this is what we found so three weeks after ovulation she was at fourteen nanogram to an ml or if you guys used an immortality she was around 45 nanometer so one thing I can tell you three weeks after ovulation I expect this number here to be much much much higher so the good thing on the good side she is pregnant but if this level of progesterone keeps decreasing she might not carry the pregnancy to term and some studies that have been done by our colleagues in Germany have shown that 25% of German Shepherd dogs of German Shepherd bitches suffering from infertility of progesterone levels that are lower than the bitches that have normal fertility so in k9 again luteal failure is essentially described in this breed and in cases like angel when we deal with history of infertility the fact that she's pregnant but we've low progesterone level at the time of the ultrasound this is typically when we will suspect this problem and the good news is that well they are alternatives that are available we can simply meant the ditch there are many called protocols that are based on the use of micronized progesterone or projected yet again progesterone analogues that we can give the bitch and can help them maintain our gestation to term because those will mimic and increase in the progesterone level in the blood of the beach and help maintain the gestation so until the bitch gives off a monitoring will be put in place to make sure that the supplementation does not need to be adjusted and that's why what we did in Angela's case we simplemente down during gestation and we stopped a few days before the expected is the expected population day she had not been pregnant before remember this time she did carry a pregnancy to term and she delivered beautiful puppies and her owner was super happy so again fortunately renal failure is not something common in canines in canine repro however if there is one weight you must know about this disorder it's definitely in German Shepherds because again this is the breed with where it is the most frequently reported and the last clinical case I want to discuss with you is about a four year old male dog named Jango so you see Jango was considered to be an elite on a great subject for his owner a very valuable breeding subject for his genetic selection program but there was a Weber a kind of a problem you see Jango as mated 25 bitches so far 25 and on those 25 none of them were pregnant not a single one so you know how this goes obviously rumors were spreading and people were saying all these don't get a problem you might not be such a good reading subject so you're not consulted with first thing to do according to you when we deal with an infant I'll meld male dog what is the first thing we should do when we have an infantile milder what's the first thing we should do to approach the situation we should perform the castration or just answer the genital tract collect and evaluate the semen or test for hypothyroidism five four three two one and I see that we all agree we need to collect the semen and evaluate the semen this is reprove 101 guys when dealing with infertility in male dog the first thing that needs to be done is to check the quality of the semen and there are three important parameters we want to monitor and evaluate here we want to check the motility the number of sperm that are moving the numeration the number of sperm in the ejaculate and the percentage of abnormal form the percentage of sperm cells that are abnormal but those are the three essential criteria we want to focus on when we check the when we do a semen analysis okay nice and motility is always again something you want to check I totally hear people telling me well Doug we've collected the semen and it was very wide you see it was like this with super wide so this is good semen qualities must be good quality because it's super wide let me tell you something it can be that wide and everything can be dead inside and no sperm cell moving so you must check the semen under microscope especially when there's a history of infertility that's the only way to make sure it is okay or not but motility is not enough sure you want to make sure that you see at least 70 80 percent of sperm cells that are moving but you also want to check two other power natures I mentioned and what about the enumeration tell me how many store methods are often in average in the male chamionship and ejaculate so I know that's a very very important data for you guys to know you will be able to brag about this when you will see other colleagues breeding other kind of dogs but again I think it's good for you to have an idea of how many tomatoes what we will find in a German Shepherd in chocolate is it 100,000 is it one to five million 200 to 400 million or 800 million to one thousand million one video sperm in the ejaculate how many sperm cells do we have in average in a male German Shepherd dog five four three two one Oh Oh it's kind of interesting we had a tie and all okay so most of you answered one to five million so you see if we have a man German Shepherd dog with one to five million scum cell you need to ejaculate now we say that this male dog is suffering from a severe illegals Australia which basically means they are not enough sperm in its ejaculate the normal German Shepherd male dog will ejaculate between 800 million to 1 billion sperm cell in its ejaculate that's the norm that's what you expect to see in a German Shepherd ejaculate that's not like a lot right but that against totally normal because in size it is its importance we need at least 150 million motile sperm to fertilize the bitch we need to have those 150 million sperm cell getting inside the uterus and because the vagina is so long there will be lots of sperm cells that will be lost and that will never reach the outside and that will never make it into the future so that's why you have so many term cell in the ejaculate of a German Shepherd dog and don't you worry this evaluation can be done pretty fast because in fact when you are used to check dog semen now you just give it a quick look under the microscope and it will immediately tell you if the numeration is okay or not because you will have a good sense or if it's you have enough sperm cell or if they have not enough of it because you always want to be above 150 million sperm cell in the ejaculate the last parameter which is super important to verify is what we call abnormal forms and this is one of the outer most important I believe because it must be observed even if sperm cells are moving well and there are enough of them because sometimes people just evaluate those two parameters motility enumeration but they don't go can be abnormal drops is there are more than fifty to forty to fifty percent of sperm cells that are defective facility might be impaired and some strong defect there are kind of obvious like those ones for instance you see this one it's a decapitate take a peek your take a figure any separation story behavior sperm cell I guess we could say this one is what we call a cold tail so basically just turning around itself so it's not able to move straight forward to reach the outside and this one has to egg when they have to as one egg is putting in one direction the other head is putting in another direction so they are just again we have this weird movement but they are not able to go and reach the eggs so those are very easy abnormality to detect and they're kind of obvious but they're only ones that sometimes are are very easily missed this one is specially called proximal cytoplasmic droplet so you see I'm going to do a quick drawing here but a sperm cell in dogs look like that and the proximal cytoplasmic droplet is basically a wrong shaped structure that will appear right here right behind the head so this is a marker of what we call testicular degeneration just that the particular tissue is aging and this would in fact decrease the motility of the edge so if you just move quickly at the sperm cell you will have lots of them they might they might have a good motility pattern but because of the presence of this droplet the the head of those chemicals are not moving properly and those sperm cells we might be able to get to the other side to get to the egg but they might not be able to penetrate the zona pellucida which is surrounding the eggs and therefore they might not be able to fertilize the eggs and in some all the dogs it's not uncommon to see that they are good motility good liberation but 90% of sperm cells with a proximal droplet and that means definitely impair the fragility potential of the animal definitely against something very important to check so you say we we saw Django and we collect the Django which honestly wasn't too difficult it was quite happy about it to tell you the truth however there was something weird you see we collected him and this is what the collection looks like so tell me guys on the chat what do you think of that this is what we collect from Django this is the ejaculate of Django what do you think of that tell me on the chest what does it look like that season Jacqueline tell me on the chest what do you think about somebody's telling it's very dense some police telling me it's kind of a weird color somebody is telling me or there's a discoloration okay you would expect human to be white or at least show the prosthetic face it will be translated the kick of the color here so you see guys this ejaculate was greenish it was married in fact it was kind of gross because this this is touch if this is what Django was ejaculating abon ejaculating since the beginning of his brilliant career before that my definitely explained the fertility issue because this is past its first in the ejaculate it's what we call by Australia what is the next step here castration x-ray you analyzes ultrasound what is the next step in general with ejaculating pus that we just highlighted what should we do here you you five four three two one so 57% of you are going for ultrasounds and you know what we will indeed do any ultrasound we would check the prostate which is the only accessory gland of the genital tract in male dogs because 90% of the volume of the ejaculate comes from the prostate and you see in Django scape this is what we saw so I again it must look like different shades of grey for you but you see this is the prostate right here beautiful but there is something weird on this prostate this thing here this big black ball it shouldn't be there this is totally abnormal we shouldn't see that and you see when we saw that we punctured it because we wanted to know what was inside we are quite a good idea of what we would find but this is what we punctured from Django's prostate this again is cus look at look at the amount of pus we were able to remove from this prostate lots of course Django was suffering from a poor static abscess and you know something might not sound right here because he has a big abscess on the prostate and abscess means first and this is the consequence of a bacterial infection but this dog is doing perfectly well he is in perfectly good health he wasn't brought to us because it was without wrinkle because he had fever or because you had difficulties to urinate or you had any urinary issues he was brought to us for fertility issues he was in perfect health and that is something you might typically observe in breeding dogs you see if we had if he had not explore and find out that you were suffering from prostatic abscess we might have developed those clinical signs you might have developed the fever the lethargy etcetera all the time but because it was a breeding dog and it was detected earlier the we could detect it earlier because it was a bringing dog and because it was into town processing disorder they are quite common in dogs in fact 80% of dogs more than 8 years of age will develop some kind of prosthetic eyes Lygia which is kind of the natural phenomenon that being said I said $80 80% of those over 8 years of age well we can find them sometimes index less than two years of age too so there's a wide range when it comes to development of the prosthetic issues the modern prostatic hyperplasia is what we will usually find more often but this prostatic hyperplasia can eventually lead to complicate lead to complication for that exist prostatic infections or prosthetic abscess and processing disorder are quite common especially in large dogs and there is one breed which is often mentioned and this breed again in German shepherds and this is what we pointed out in this study we did back in 2011 so if we go back to general yes is suffering from the prostatic upset what about is reading career tell me on the child yes no is it order or not can we still consider breeding him in the future do you think what do you think about in breeding career is it over up look tell me on the chest some Emily is telling me I think he has a future she hasn't seen the presentation yet gap not over not over not over so we all agree his breeding career is another of course if it was although I won't be able to finish my story right one essential element of prosthetic disorders is a hormonal component the secretion of testosterone so the best way to remove this the best way to remove this assailants equation would be to castrate the dog but this is a valuable breeding though we are talking about so there must be something else that can be done and in fact we have medical protocols today that can help us remove the effect of testosterone on the prostate while at the same time not impairing fertility those many controls of course will lead to a decrease in the size of the prostate which is what is needed in fact to for the prostate to be cure so to speak and in Django's case might not be enough because here is suffering from the prostatic abscess there is a bacterial infection going on as well antibiotics will be required you see in those in this plus root puncture we culture of E coli so we this is something we might find in the vagina of the bitch but this is definitely abnormal inside the prostate and we will need to give him antibiotics and we will need to cure the abscess we will need to truly abscess so this can be done by surgery or this can also be done by ultrasound guided aspiration which is what we did in his case because we are the fantastic radiologist who really was very good at puncturing those cavities so we were able to treat it and inject directly inside the util inside the body upset antibiotics to cure it faster when we had fantastic results in Jango scale and we did a study when I was at the vet school where we studied in fact fertility in the email that suffering from prostatic disorders and when we were able to identify prostatic disorder and treat them we were able to restore fertility in nearly 60% of the cases so infertility in male dogs is always of bad prognosis but if we can find out what's going on and if we have the appropriate medical treatment you see that we can see activity can still be recovered in to 60% of the case which is already good success rate in my opinion you so you see we did the treatment and Django was to be reassessed few months after no need to do it earlier because in dogs the scrum cycle lasts around 60 days so any time a dog is thick there is no point controlling the steamin until two months after the end of the medical treatment this is only when you will get infected clear you will reach a clear conclusion so a few months after I saw Django again collected the semen and guess what it was perfect could not see DT good enumeration less than 20% of normal abnormal forms perfect perfectly adequate semen which was totally compatible with fertility so again protecting these daughters are very common in large dogs but there is over a clear predator edition of general for German shepherds to develop this problem even early during their lifetime you definitely need to have an idea of what this is about because this is something again you might run into in this grid for sure I'm quite sure about that and we reach the end just covered three clinical cases and you see Tonya and Joel Jango they all displayed some typical problems we encounter especially in German Shepherd dogs and it's like in all canine breeds you know they all have their predispositions to this and to that but one of my favorites a an investment in knowledge always pays the best interest you see tonight you learned about some of those issues today and how we solve them now I hope that you'll be better prepare if you ever have to face them and that's what I wanted to tell you tonight guys so thank you very much for participating to this webinar today it's always a pleasure to spend some time with you guys online so I'm not going to stop the recording but hey don't leave us now because it's central question is time for Q&A so if you have questions it is now or never so turn them to the chat and we will answer as many as we can so please fire em up I am ready for that and again if you need to go really want to thank you for participating always a pleasure I recognize a few names here hello to my friends from Kuala Lumpur in Malaysia always a pleasure to see you guys here I get it morning for you but thank you very much always a pleasure and thanks for all this interactivity and engagement online always a pleasure to do those webinar thank you guys and if you have questions put them on the chat

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